services

HumanZyme is a world leader in human protein expression and purification services in human cells. It distinguishes itself as the leader in medium or large scale recombinant human protein production in human cells.  We have a proven technology, HumaXpress ™, to produce proteins that have been economically unviable to produce in the past. HumanZyme offers complete solution for your needs, including: identifying human genes, generating expression construct, and expressing and purifying proteins in small and large scale.

1. Expression construct
• Identify human gene sequence
• Order or synthesize human gene
• Subclone gene into our proprietary expression vectors.
2. Small scale expression and purification
• Optimize transfection condition
• Express and purify the protein in small-scale.
3. Establish high-expression stable cell line
4. Scale-up transient or stable expression and protein purification
• Grow the culture.
• Express and purify the protein.
5. Purification options
• Protein purification by affinity column
• Protein purification by other columns including gel filtration, ion exchange and hydrophobicity chromatography
• Remove the purification tag by a protease, and purify the protein by gel-filtration.
6. Large scale and GMP or GMP-like manufacturing

Quotation and Order
Please email info@humanzyme.com about your project goals, and we will send you our competitive quotation shortly. For quotation request, please include the following information:

1. Name and source of the gene or protein
2. Sequence of the gene or protein.
3. Expression with or without  an affinity tag
4. Quantity of protein and timeline