G-CSFHuXp - More Effective For Human Cell Growth Stimulation Authentic - Human Cell Expressed
Introduction
Cytokines are a group of proteins and polypeptides
that organisms use as signaling molecules. Most
cytokines are glycoproteins less than 30 kDa in
size and bind to specific, high-affinity cell surface
receptors. Due to their central role in the
immune system, cytokines are involved in a variety
of immunological, inflammatory and infectious
diseases and widely used in research, diagnostics
and therapeutics. Cytokines generally alter the
gene expression pattern of the target cell which
leads to changes in the rate of cell proliferation
and/or in the state of cell differentiation. Currently,
these proteins are predominantly produced in
non-human cells (e.g. E. coli) and therefore lack
authenticity due to the absence of physiologically
relevant glycosylation. In addition, a number of
important cytokines are not commercially available
due to inadequate proteolytic processing, protein
folding or other post-translational modifications
that occur in the non-human cell expression
systems.
HumanZyme has developed an efficient humancell
based technology, HumaXpress™, for costeffective
and scalable production of human
cytokines. We have an expanding range of tag-free
cytokines, including difficult-to-express members
of the TGFβ superfamily. As demonstrated
below, HumanZyme’s authentic cytokines can
be used as highly preferred reagents for stem
cell, cancer, inflammation research, and antibody
development.
G-CSFHuXp
Granulocyte colony-stimulating factor (G-CSF), is
a hematopoietic growth factor that stimulates the
development of committeed progenitor cells to
neutrophils and enhances the fundctional activities
of the mature end-cell. Currently, commercially
available G-CSF proteins are produced in an E coli
expression system and typically have a molecular mass of 19kDa. HumanZyme Inc. scientists have
produced G-CSFHuXp expressed in engineered
human 293 cells. The proteins are glycosylated
forms with a molecular mass range of 22-25 kDa.
G-CSFHuXp can be cost-effectively produced at
large scale.
The bioactivity of G-CSFHHuXp was determined
by the dose-dependent stimulation of the
proliferation of murine M-NFS-60 cells. G-CSF
expressed in E. coli was compared to the human
cell expressed version G-CSFHuXp. The results
indicate that G-CSFHuXp is 4-5 fold more active
than the E coli expressed cytokine and is more
effective for stimulating cell growth.
(View more information for product number HZ-1016, HZ-1017, HZ-1018 and HZ-1050.)
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