Authentic Activin HuXp Expressed in Human Cells
Introduction
Cytokines are a group of proteins and polypeptides that organisms use as signaling molecules. Most cytokines are glycoproteins less than 30 kDa in size and bind to specific, high-affinity cell surface receptors. Due to their central role in the immune system, cytokines are involved in a variety of immunological, inflammatory and infectious diseases and widely used in research, diagnostics and therapeutics. Currently, these proteins are predominantly produced in non-human cells (e.g. E. coli, SF9, CHO) and therefore lack authenticity due to the absence of physiologically relevant glycosylation. In addition, a number of important cytokines are not commercially available due to inadequate proteolytic processing, protein folding or other post-translational modifications that occur in the non-human cell expression systems. HumanZyme has developed an efficient human-cell based technology, HumaXpress™, for the scalable production of human cytokines. The company is expanding this range of tag-free produced cytokines, including difficult-to-express members of the TGFβ superfamily. HumanZyme™ authentic cytokines are preferred reagents for stem cell, cancer, inflammation research, and antibody development.
Activin AHuXp
Activin A belongs to the TGF-β super-family and exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Activin A is a disulfide-linked dimeric protein produced as precursor with an amino-terminal propeptide that is enzymatically cleaved to release the carboxy-terminal bioactive ligand. Due to complex post-proteolytic modification processes, yield from insect cell and CHO cell expression systems is low and as a result bulk volume is uneconomical and not readily available. HumanZyme has produced Activin AHuXp in a stable cell culture of engineered human HEK293 cells. The protein is expressed as a disulfide-linked dimer of 26 kD and, due to the scalability of the stable culture, can be cost-effectively produced. As shown In Fig. 1, Activin AHuXp is highly purified while a commercially sourced cytokine expressed in CHO exhibits contamination by the precursor and several other proteins.
Activin AHuXp was assayed by its ability to inhibit the proliferation of murine MPC-11 cells. The results indicate the activity of HumaXpress™ Activin A is comparable to that of the CHO expressed version. HumanZyme cytokines are manufactured to high quality standards and provide high biological activity, lot-to-lot consistency and low endotoxin levels.
(View more information for product number HZ-1136, HZ-1137 and HZ-1138.) Activin AHuXp is available in trial size and in bulk.
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